At a packed Victoria Theatre in San Francisco, Glover said Sunday that the work Williams has done behind bars is an "example that breathes life not only into gang members but into us."
The event, a fund-raiser for the "San Francisco Save Tookie Coalition," included a speech by Barbara Becnel, a co-author to Williams' children's books about the tragedy of gang life.
The night ended with a screening of the movie "Redemption: The Stan Tookie Williams Story," in which actor Jamie Foxx plays Williams.
Williams, a co-founder of the Crips gang, is sentenced to die by lethal injection Dec. 13 for murdering four people in 1979.
A hearing to determine whether Gov. Arnold Schwarzenegger will grant Williams clemency is scheduled for Thursday.
"The work is still there to do," Glover said. "Yes, Tookie Williams is in our hearts today, and we must demand that Tookie Williams' voice is heard."
The 200 people crowded inside the theatre did hear Williams' voice when a cell phone was put up to a microphone and Williams started speaking.
"Can you hear me?" he asked. The group screamed "Yeah!" in unison and then erupted into applause that drowned out Williams' voice.
After the applause dissipated, Williams thanked the crowd for being there and staying "true" to him. "Even those who don't believe in the love of God could feel the warmth ... that you have given tonight," he said.
After Williams' surprise phone call, cinematographer Kevin Epps stepped up to the lectern with tears in his eyes.
"We gotta save this brother's life," pleaded Epps, best known for his documentary "Straight Outta Hunters Point," a film about poverty and devastated lives prevalent in San Francisco's Hunters Point.
"If we can save (Williams), we save a generation," Epps said. "This is the truth. For real."
"Tookie Williams must live," Glover said at the end of his speech. "(This) will define us as human beings."