Toronto manager John Gibbons used his challenge to ask umpires to review a play in the sixth inning of Monday's game against Minnesota.
Chris Rahl of the Twins was called safe at first base when shortstop Munenori Kawasaki's throw pulled Jared Goedert off the bag.
The umpires gathered in front of the first base dugout while umpire Brian O'Nora watched the replays from a satellite truck outside the ballpark. After a wait of 2 minutes, 34 seconds, first base umpire Fieldin Culbreth spread his hands in the safe sign.
MLB has expanded replay this year beyond potential home runs and boundary calls. This season, most every play except for balls-and-strikes is subject to a review, with calls coming from a replay booth in New York.